|
KMID : 0545120080180101659
|
|
Journal of Microbiology and Biotechnology
2008 Volume.18 No. 10 p.1659 ~ p.1662
|
|
|
Identification of an ISR-Related Metabolite Produced by Pseudomonas chlororaphis O6 Against the Wildfire Pathogen Pseudomonas syringae pv.tabaci in Tobacco
|
|
Park Myung-Ryeol
Kim Young-Cheol
Park Ju-Yeon
Han Song-Hee
Kim Kil-Yong
Lee Sun-Woo
Kim In-Seon
|
|
|
Abstract
|
|
|
|
Pseudomonas chlororaphis O6 exhibits induced systemic resistance (ISR) against P. syringae pv. tabaci in tobacco. To identify one of the ISR metabolites, O6 cultures were extracted with organic solvents, and the organic extracts were subjected to column chromatography followed by spectroscopy analyses. The ISR bioassay-guided fractionation was carried out for isolation of the metabolite. Highresolution mass spectrometric analysis of the metabolite found C9H9O3N with an exact mass of 179.0582. LC/MS analysis in positive mode showed an (M+H)+ peak at m/ 180. Nuclear magnetic resonance (1H, 13C) analyses identified all protons and carbons of the metabolite. Based on the spectroscopy data, the metabolite was identified 4-(aminocarbonyl) phenylacetate (4-ACPA). 4-ACPA applied at 68.0 mM exhibited ISR activity at a level similar 1.0 mM salicylic acid. This is the first report to identify an ISR metabolite produced by P. chlororaphis O6 against the wildfire pathogen P. syringae pv. tabaci in tobacco.
|
|
|
KEYWORD
|
|
|
Induced resistance, ISR, Pseudomonas chlororaphis O6, Pseudomonas syringae pv. tabaci
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|